pCMV-myc pCMV-myc-N或pCMV-myc-C 包邮上海生物网瑞楚生物嘉楚生物

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出品公司: Clontech
载体名称: pCMV-myc
质粒类型: 哺乳细胞表达系统载体
高拷贝/低拷贝: --
启动子: CMV
克隆方法: 多克隆位点，限制性内切酶
载体大小: 3791bp
5' 测序引物及序列: pCMV：GATCCGGTACTAGAGGAACTGAAAAAC
3' 测序引物及序列: --
载体标签: Myc (N-term) (MASMQKLISEEDL)
载体抗性: Ampicillin
筛选标记: --
备注: 用于哺乳细胞表达N端带有Mcy标签的蛋白
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

LOCUS       pCMV-Myc 3791 bp DNA SYN
DEFINITION pCMV-Myc
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
COMMENT     VNTAUTHORNAME
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="pCMV-Myc"
                     /mol_type="other DNA"
     misc_feature    31..318
                     /label="CAG_enhancer"
     promoter        36..528
                     /label="CMV_immearly_promoter"
     misc_feature    485..505
                     /label="CMV_fwd_primer"
     promoter        486..555
                     /label="CMV_promoter"
     promoter        498..617
                     /label="CMV2_promoter"
     misc_feature    529..548
                     /label="pCEP_fwd_primer"
     misc_feature    531..555
                     /label="LNCX_primer"
     misc_feature    631..657
                     /label="pCMV_primer"
     misc_feature    672..768
                     /label="modSV40_late_16s_int"
     misc_feature    672..702
                     /label="SV40_late_19s_int"
     misc_feature    832..855
                     /label="Myc_primer"
     terminator      complement(940..1059)
                     /label="SV40_PA_terminator"
     misc_feature    complement(954..973)
                     /label="EBV_rev_primer"
     promoter        complement(1171..1189)
                     /label="M13_reverse_primer"
     misc_feature    complement(1188..1210)
                     /label="M13_pUC_rev_primer"
     promoter        complement(1224..1253)
                     /label="lac_promoter"
     gene            complement(2336..3196)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2336..3196)
                     /label="ORF frame 2"
     promoter        complement(3238..3266)
                     /label="AmpR_promoter"
     misc_feature    complement(3425..3447)
                     /label="pGEX_3_primer"
     misc_feature    complement(3646..3789)
                     /label="lacZ_a"
     misc_feature    3760..3782
                     /label="M13_pUC_fwd_primer"
     promoter        3775..3791
                     /label="M13_forward20_primer"
ORIGIN
    1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
   61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GGCATCAATG
841 CAGAAGCTGA TCTCAGAGGA GGACCTGCTT ATGGCCATGG AGGCCCGAAT TCGGTCGACC
901 GAGATCTCTC GAGGTACCGC GGCCGCGGGG ATCCAGACAT GATAAGATAC ATTGATGAGT
961 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG
1021 CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA
1081 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTCGGAT CCTCTAGAGT
1141 CGATCTGCAG GCATGCTAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT
1201 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG
1261 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT
1321 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT
1381 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC
1441 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG
1501 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
1561 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
1621 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
1681 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
1741 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
1801 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
1861 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
1921 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
1981 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
2041 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
2101 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
2161 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
2221 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
2281 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
2341 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
2401 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
2461 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
2521 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
2581 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
2641 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
2701 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
2761 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
2821 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
2881 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
2941 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
3001 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
3061 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
3121 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
3181 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
3241 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
3301 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA
3361 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG
3421 AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG
3481 GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA
3541 ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC
3601 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT
3661 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT
3721 GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA
3781 CGACGGCCAG T